Thank you for joining us at CYTO 2010 (ISAC)

Workshop:
Panel Design: Getting More "Multi" Into Your Multi-Color

Presenter:  William Godfrey, Ph.D., Manager, Reagent and Application Development, Beckman Coulter, Inc The three Beckman

Workshop Description:
In recent years flow cytometers have become more powerful with the addition of large laser palates and multiple flourochrome options. This talk will look at the considerations a researcher needs to make while designing high color applications. We will look at the importance of titration, spectral overlap, dye selection, tandem dyes, background control and sample prep when constructing a complex cocktail or panel. Specific panel and cocktail examples to be discussed include L/L, Cell Signaling and NK. We will also examine how any new dyes will effect panel development.

Workshop:
High Speed, High Color Six-way Sorting of Circulating Hematopoietic Progenitor Cells and Lymphocyte Subsets

Presenter: Chris Novak, Global Product Manager, Flow Cytometry, Beckman Coulter, Inc.

Workshop Description:
The Beckman Coulter MoFlo™ Astrios™ sorter is a high-end 7-laser system which allows for the ultimate in cell sorting flexibility. In this tutorial we will illustrate the system's features looking at bio containment, automation, flexibility and operator ease-of-use. Cells samples analyzed will include Circulating Hematopoietic Progenitor Cells and a comprehensive sort of T Cell subsets. The data we review demonstrates the performance of the system with respect to, six-way sorting, sensitivity, sort speed, purity and recovery of sorted populations.

Workshop:
New Frontiers in High Sensitivity Cytometry

Presenter:  Sharlene Wright, Flow Cytometry Strategic Marketing Manager, Beckman Coulter, Inc

Workshop Description:
Through innovations in optical and electronics design, cytometers have progressively improved their ability to reliably resolve very small particles and dimly fluorescent signals. The Beckman Coulter Gallios system employs short optical paths and high power lasers, delivered directly through air to increase the available excitation energy. Additionally, highly efficient collection optics are used to ensure that the emitted light loss is minimized.

Complementing these innovative optical designs is the highly resolved electronics that use a 20 bit integral signal and 40MHz sampling rate. The Gallios is therefore able to maintain very high sensitivity without compromising the speed of data acquisition. Other innovations, such as the collection of two angles of forward scatter, allows for much greater resolution between sub-micron particles. The benefit of the application of these technologies is clearly evidenced by the superior resolution in dimly fluorescent bead populations that has been reproducibly demonstrated in comparison with a variety of other multi-color cytometers. Of course beads are just beads and unless these benefits are manifest in cellular analysis, they are of limited value to the cytometrist. Fortunately, a variety of enhanced cellular applications have been described that have been enabled or significantly improved using the Gallios system. Examples include the improved size resolution of both red cell microvesicles and platelet microparticles.

 In the context of fluorescence detection, applications that include vector transfection confirmation and complex cell signaling assays, were difficult using other systems have been enabled by the fluorescence resolution of Gallios. Finally, the speed of the Gallios expressed in events per second has been studied in comparison with other systems. It is clear, that while some systems suffer major reductions in the yield of detectable events, the Gallios is able to markedly reduce cellular data losses, even as the maximum event rate of 25000 EPS is reached. These features of more highly resolved size and fluorescence signals, as well as high speed and yield in a 10 color flow cytometer, have resulted in tangible benefits in the performance of cytometric applications and in some instances, wholly new applications. 

Workshop:
Simultaneous Detection of Five Fluorescent Proteins: Customization of a Beckman Coulter Cyan Flow Cytometer

Presenter:  E. Michael Meyer

Workshop:
Laser Scanning Cytometry. Where does it “ fit in” with modern biomedical imaging?

Presenter:  Bill Telford, Ph.D.

Workshop Description:
Image cytometry (also termed laser scanning cytometry) represents a unique hybrid technology between flow cytometry and traditional fluorescent microscopy. Imaging cytometers analyze the light scattering and fluorescent properties of biological specimens like a flow cytometer, but also collect correlated quantitative images of the cells simultaneously. This powerful technique is a dramatic expansion of traditional cytometric analysis, allowing the cell images and their morphological and structural information to become a cytometric parameter, like forward scatter or fluorescence. In this introductory talk, we will discuss the theory and practice of image cytometry and provide a general overview of the technology available to biomedical scientists. We will also cover a wide variety of applications for this technology, from individual cells to intact tissues and whole organisms.