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Flow Cytometer - Analyzer

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Instrument

The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays.

Features

The Gallios houses up to three highly reliable, solid-state lasers in standard red and blue, with violet available as an option. Easily interchanged optical filters facilitate detection of a variety of dyes and wavelengths.

An innovative forward-scatter detector provides up to three measurements of cell size and visualization of particles down to 0.404 µm in diameter. A side-scatter detector incorporates an independently focused, high-performance photodiode with electronic attenuation. Six fluorescence detectors provide simultaneous acquisition of up to six fluorescence signals. Up to four fluorescence detectors can be added to the system, enabling concurrent reading of up to 10 colors. Simultaneous measurements of integral, peak and width are available for all parameters, including scatter and fluorescence signals. A selection of up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second, with high yield. Three scalable configurations allow for the future addition of more lasers and fluorescence detectors as the applications for complex multi-color flow cytometry expand.

Available in 3 configurations
6 color 2 Laser (5+1)
8 color 2 Laser (5+3)
10 color 3 Laser (5+3+2)

Innovative laser cartridges
Picomotor driven beam steering optics
Laser control to conserve laser life
Design for laser flexibility
Customer temperature regulation

Plug and play filters allow rapid instrument reconfiguration between experiments
Filters are easily accessible and interchangeable with no tools required

Software features:
Multi-User Administration
Drag & Drop replay of data
Boolean gating and color eventing hierarchy
New log/lin transform for visualization of negative data and compensation optimization
Automatic PDF and .xls creation

Instrument Dashboard
LEDs clearly indicate instrument status from across the lab

Efficient delivery of light
More power at the flow cell for greater resolution in your applications
Efficient and reliable solid state blue, red, and violet lasers
Free space delivery of laser light
Optimized beam steering optics
Increased power at flow cell

Scatter Parameters
Efficient delivery of light
3 unique setting for relative size measurements
Innovative submicron particle resolution setting for forward scatter
Independent side scatter alignment

Patented electronics design incorporates fast, accurate measurement of signals over a wide range of event rates
Fast pulse recovery
Minimize linearity errors
Sensitivity of dim particles
Minimize dead time
Accuracy at high event rates

PROService Compatible


Literature

For other Gallios related documentation visit the Knowledge Base Document Library section

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Part Number

Document View

2

A75199AA Quick Reference Guide

Recent citations and publications

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Citation

PubMed - U.S. National Library of Medicine NIH Link

1

Blood.
2011 Jan 13
Normal bone marrow signal transduction profiles: a requisite for enhanced detection of signaling dysregulations in AML

2

Journal of Thrombosis and Haemostasis 8:2571-2574 Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop

Clinical and Vaccine Immunology,
January 2011, p. 125-134, Vol. 18, No. 1
Human Leukocyte Antigens A*3001 and A*3002 Show Distinct Peptide-Binding Patterns of the Mycobacterium tuberculosis Protein TB10.4: Consequences for Immune Recognition

4

FEMS Immunology & Medical Microbiology DOI: 10.1111/j.1574-695X.2010.00777.x Oral administration of live Lactococcus lactis C59 suppresses IgE antibody production in ovalbumin-sensitized mice via regulation of interleukin-4 production

Rheumatology (Oxford). 2010 Dec 23 HLA-G and HLA-E in patients with juvenile idiopathic arthritis

6

Cytometry A
2010 Jun
77(6):502-14
Flow cytometric analysis of circulating microparticles in plasma

7

Blood
2010 May 6
115(18):3843-51
Soluble CD146 displays angiogenic properties and promotes neovascularization in experimental hind-limb ischemia

8

Oncogene
2010 Apr 8
29(14):2047-59
TrkB induces EMT and has a key role in invasion of head and neck squamous cell carcinoma

9

Hæmatologica
2010 Sep 7
Atypical familial hemophagocytic lymphohistiocytosis due to mutations in UNC13D and STXBP2 overlaps with primary immunodeficiency diseases.

10

American Journal of Transplantation
10(9):1981-1990
Rapamycin and MPA, But Not CsA, Impair Human NK Cell Cytotoxicity Due to Differential Effects on NK Cell Phenotype

11

Cancer Immunol Immunother
2010 Sep 21
Fludarabine modulates composition and function of the T cell pool in patients with chronic lymphocytic leukaemia.

12

Brain Behav Immun. 2010 Oct 22. Both MHC and non-MHC genes regulate inflammation and T-cell response after traumatic brain injury

13

J Thromb Haemost. 2010 Sep;8(9):2042-52. Tissue factor up-regulation in proinflammatory conditions confers thrombin generation capacity to endothelial colony-forming cells without influencing non-coagulant properties in vitro.

Consumables

Click on the shopping cart icon to purchase the consumables specifically designed for use on the Gallios.

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Detail

Part Number Name View

1

6607077

IMMUNO-TROL™ Cells Add to cart

2

6604248

CYTO-TROL™ Control Cells Add to cart

3

6607023

CYTO-COMP™ Cell Kit Add to cart

4

8546859

IsoFlow™ Sheath Fluid, 10 L Add to cart

5

177017

QuickCOMP 4 Kit Add to cart

Parts

Click on the shopping cart icon to purchase parts and accessories specifically designed for use on the Gallios.

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Detail

Part Number Description View

1

775016

Gallios 10 colors, 3 lasers (5+3+2 configuration) Add to cart

2

775014

Gallios 6 colors, 2 lasers (5+1 configuration) Add to cart

3

775015

Gallios 8 colors, 2 lasers (5+3 configuration) Add to cart

4

A82959

Single-use license through USB dongle Add to cart

5

A84174

One year single-use license through USB dongle Add to cart

6

A85788

3 User bundle (Full Version) Add to cart

A82960

5 User bundle (Full Version) Add to cart

8

A84175

5 User Network (Full Version) Add to cart

9

A82961

10 User Network (Full Version) Add to cart

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775016 - Gallios 10 colors, 3 lasers (5+3+2 configuration)
775015 - Gallios 8 colors, 2 lasers (5+3 configuration)
775014 - Gallios 6 colors, 2 lasers (5+1 configuration)
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For Research Use Only. Not for use in diagnostic procedures


Designed to fit your needs, today and tomorrow, the Gallios flow cytometer provides efficient acquisition of superior quality data from up to 10 colors with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve the most accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was designed for your research needs.

Gallios is optimized for unparalleled signal to noise resolution in all parameters and its quick and easy setup of automated walk-away processing improves your workflow while its remote diagnostics minimize downtime and allow you to maximize lab productivity.

Developed for the research and biopharmaceutical markets, with a  particular emphasis on optical and electronics design, combined with powerful automation and software tools, allows ly enhance the efficiency and analytical capabilities of flow cytometry laboratories. Patented electronics provide accurate and efficient digital signal processing at high event rates over a wide dynamic range of fluorescence intensities. These benefits are packaged in a compact analyzer that delivers stable performance over long periods of time and across a wide range of operating temperatures.

 
Features Literature Consumables Parts and Accessories

Available configurations
ProService Compatible

IFU
Quick reference guide

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