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Flow Cytometer - Analyzer

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Instrument

FC 500 Series More Flexibility for Your Cytometry Research, the FC 500 Series is a sophisticated product family equipped with the most advanced technologies available.

FC 500 MCL System
FC 500 MCL System with CXP Software

The FC 500 System provides automated tube based acquisition for cell based assays. Utilizing powerful CXP software, investigators can create custom panel reports and perform multiple tube panel based analysis. The automated QC database allows daily monitoring of instrument QC.


FC 500 MCL System
FC 500 MPL System with MXP Software

The FC 500 MPL System provides automated plate and tube based acquisition for cell and bead based assays. MXP software allows users to automatically setup assays and rapidly run samples in Microtiter plates for screening and higher throughput analysis.

Features

Precise, Flexible Analysis Every Time
Designed for powerful performance and unmatched simplicity Conducts 5-color analysis from either single or dual laser excitation Improves efficiency by enabling scientists to analyze more antibodies per tube than ever before on an automated system Gives investigators the power to expertly manage every aspect of their flow cytometry studies with the revolutionary CXP software.

Increased Flexibility with Two Lasers and Multiple Fluorochromes
The systems have the capacity to conduct 5-color analysis using either asingle(488nm)laser or adual(488nm/633nm)air-cooled laser configuration. Coupled with the ability to perform tube or plated based automated analysis, the FC500 Series improves efficiency by enabling users to easily manage and automate every aspect of their flow cytometric analysis.

Simplified Multicolor Analysis with Advanced Digital Compensation (ADC)
Beckman Coulter's ADC technology provides superior correction of spectral overlap and more accurate results. With ADC technology, biological samples can be used for automated color compensation, without the limitations of latex beads. Only Beckman Coulter flow cytometers offer patented Digital Signal Processing (DSP) for excellent linearity, drift-free amplification and color compensation.

The FC 500 with ADC also enables investigators to:
• Perform digital signal processing with 20-bit data in real-time
• Obtain fast, intuitive compensation feedback to set or fine-tune compensation manually with the QuickCOMP
  feature
• Increase the visualization of low intensity fluorescence populations

To accommodate numerous configurations, the FC 500's user changeable filters provide fluorochrome selection flexibility. Customized Photomultiplier Tubes (PMTs) detect a broad range of wavelength emissions, which can be used for virtually any dye excited by 488 or 635 nm lasers.

Five-Color Analysis From A Single Laser Has Never Been Easier
Only the FC 500 can be used with a variety of fluorochromes to analyze five colors (FITC, PE, ECD, PC5 or APC and PC7) using either one or two lasers. Investigators could also use Beckman Coulter's IOTest multicolor reagents and drop in their choice of additional conjugated antibodies to make their own combinations. Performs five-dimensional automated phenotyping analysis with Prism, a unique data reduction display allowing analysis of up to 32 individual phenotypes in a single plot. Extends multicolor analysis to utilize the full visible emission spectra, which further boosts the level of flexibility that can be achieved

Literature

For other FC 500 related documentation visit the Knowledge Base Document Library section

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Part Number

Document View

1

722238 Quick reference Guide

2

DS-9812D Cytomics FC 500 Flow Cytometry System - Data Sheet

3

BR-9543A Cytomics FC 500 MPL Flow Cytometry System with MXP Software Brochure

BR-10212A Immunotox Three-Color Combinations: iTox3

5

BR-10907A ImmunoTox for Non-Human Primate Lymphocyte Phenotyping

6

DS-10629A Instrument Manager Version 8.05 with Bidirectional LIS Connectivity for FC 500 Systems

7

A-10954A Fundamentals of Standardization for Flow Cytometric Measurements

8

175570 Reference

9

175572 Special Procedures and Troubleshooting

10

177652 Immunotox Three-Color Combinations: iTox3

Consumables

Click on the shopping cart icon to purchase the consumables specifically designed for use on the FC 500.

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Detail

Part Number Name View

1

8546929

COULTER CLENZ® 500 mL Add to cart

2

8546930

COULTER CLENZ® 5 L Add to cart

3

8546931

COULTER CLENZ® 10 L Add to cart

4

6605359

Flow-Check™ Fluorospheres 3 x 10 mL IVD & CE Add to cart

A70449

FlowClean Cleaning Agent, 500 mL Add to cart

6

7547053

Flow-Count™ Fluorospheres, 1 x 20 mL Add to cart

7

6607007

Flow-Set™ Fluorospheres, 3 x 10 mL Add to cart

8

8546859

IsoFlow™ Sheath Fluid, 10 L Add to cart

Parts

Click on the shopping cart icon to purchase parts and accessories specifically designed for use on the FC 500.

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Detail

Part Number Description View

1

623101

Red Diode Laser Upgrade Add to cart

2

733321

MPL Upgrade Add to cart

3

773875

Offline Analysis Workstation Add to cart

Software

Click on the shopping cart icon to purchase parts and accessories specifically designed for use on the FC 500.

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Detail

Part Number Description View

1

 

Request quote

tetraCXP SYSTEM Software  

2

 

Request quote

stemCXP SYSTEM Software  

3

 

Request quote

CXP Analysis Software Packages  

4

6415063

MultiCycle for Windows Add to cart

FC 500 with Carousel loader and CXP software

AUTOMATED QUALITY CONTROL MONITORING
To comply with the rigors of the routine flow cytometry laboratory, CXP software provides automatic daily monitoring of system performance, simplifying daily quality control recording. Built in Levy-Jennings plots are updated with each QC run for constant quality monitoring. The integrated product log keeps track of lot numbers and expiration dates helping with compliance for regulated laboratories.

MULTI-FILE ANALYSIS SOFTWARE
Data files produced by the FC 500 Flow Cytometer contain complex biological information. Review of the data off line to acquisition is an important process. The CXP software provides powerful tools that offer flexibility and automation in data analysis. It is possible to analyze multiple files concurrently or on one desktop to glean as much information as possible from an experiment or patient sample. Overlay histograms can be created to visualize small changes in populations together with three dimensional plots to analyze interacting parameters. All of which can be processed manually or in batch mode.

CUSTOMIZED PANEL REPORTING
Available within CXP software to tailor reports to groups of assays. Each report can include both user selected statistics as well as plots from the panel. Output can be made via a printed page or electronic PDF documents to easily e-mail to off site laboratories.

FC 500 MPL with MXP software
Accepts a variety of microtiter plates and 12 x 75 mm tubes.

MXP's 21 CFR PART 11 TOOLS
Provide the most complete set of optional tools to facilitate compliance for regulated flow cytometry laboratories, giving the maximum protection of data integrity and version tracking. Sub systems such as inactivity timeouts and attempted failure prevention allow only those with verified access rights to gain entry to the software. This combined with E signatures and the Audit Trail tracking of produced Microsoft Excel files protects output from the system.

MXP's PLATE WIZARD
Improves usability and simplifies plate setup, allowing the user to rapidly create work lists for plate based analysis including multi-well panel, protocol and sample ID assignments. For flexible choices of sample types simply select sample volume and mix requirements, then store plate parameters for rapid retrieval.

AUTOMATED ANALYSIS TOOLS FOR BEAD BASED ASSAYS
Multiplexed ELISA assays utilizing Micro-beads are now acommon application in flow cytometry. Software modules built into the MXP software enable the FC 500 MPL to carry out these assays with ease. Data from acquisition is automatically exported to Microsoft Excel then analyzed using specifically designed MPL analysis macros. Well plot graphs display well data on a macro level enabling easy visualization of "hits" onan assay by assay basis. Also included is the automatic generation of standard curves for determination of analyte concentration.


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FC 500 MCL System with CXP Software
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Instruments | Features | Literature | Consumables | Parts | Software | Request Quote
For Research Use Only. Not for use in diagnostic procedures


The Cytomics FC 500 Series is a sophisticated product family equipped with the most advanced technologies available. The FC 500 Series delivers flexible testing, powerful software and a user-friendly interface - features that help scientists optimize their overall workflow efficiency while maintaining true cost effectiveness. Plus, each FC 500 Series system is backed by Beckman Coulter's unsurpassed reputation for quality and service.
 
Features Literature Consumables Parts and Accessories

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Five-Color Analysis

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