Laser Scanning Cytometry (LSC)
uses laser-based opto-electronics and automated analysis capabilities to simultaneously and rapidly measure biochemical constituents and evaluate cell morphologies. LSC technology provides a measure of precision and visualization, combined with flexibility that is available in no other cellular analysis system.
iCys® Research Imaging Cytometer
iCyte® Automated Imaging Cytometer
Features
Although LSC technology has a flow cytometry heritage, it is not limited to analyzing cells in fluids. Instead, the technology allows automated analysis of solid-phase samples, including adherent cultured cells, tissue sections, cancer tissue imprints, and cytology smears, preserving the sample along with the exact position of each measured sample. This important feature allows the researcher to automatically return to, visually inspect and interrogate specific cells having defined genetic, biochemical, or morphological properties; or to remeasure specimens after retreating them with reagents or drugs.
Laser Scanning Cytometry allows users to:
· Generate stoichiometric data and analyze heterogeneous cell populations.
· Visualize specimens conventionally with either powerful laser-scanning imaging techniques or with a microscope.
· View any number of individual events in the population data.
· Reanalyze the same cells under varying conditions.
· Measure and localize cellular constituents.
· Detect molecular constituents in the surrounding environment and correlate their presence to cell processes.
· Simultaneously study cultured cells at the individual- and colony-level.
· Perform automated analysis on tissue sections and tissue microarrays.
iCys® Research Imaging Cytometer
· Simultaneous acquisition of multiple-fluorescence and brightfield laser-scatter images, from as many as 3 lasers and 5 multiplexed light sensors
· Wide-field images, super-wide-field sampling and mosaic images
· Easily viewed specimens with on-the-fly galleries, or relocation to individual events after data acquisition
· The option for either laser scan images or live microscope or camera viewing, or both
· Analysis and visualization of specimens in a variety of formats (chamber slides, microtiter plates, petri dishes, microscope slides) to analyze cell populations, cell colonies, tissues and tissue arrays
· Precise localization of cells and tissue events of interest with the automated motorized stage
· Autofocus for automated analysis
· Easy interface with peripheral microscopy devices like cameras, phase-contrast visualization, micromanipulators, cloning devices, etc
iCyte® Automated Imaging Cytometer
· Morphological and quantitative features for very high content cell-based analysis
· Multiple lasers and photomultipliers for flexible dye combinations
· Compatibility with a variety of carriers, including microtiter plates and microscope slides, for unsurpassed specimen flexibility
· Automated or manual operation modes
· Cellular and tissue specimen analysis
· Open assay development and data reporting platforms to accommodate rapidly changing discovery needs
iCyte enables fully automated, walk-away analysis of cellular specimens. New features include:
· Robotic specimen loader with 45-tray capacity, allowing walk-away experimental analysis
· Optional iNovator™ Application Development Toolkit, enabling fully automated tissue identification and scan region selection. Accurate quantitative measurement of tissue fluorescence is enhanced by novel background fluorescence subtraction techniques
· Flexible iBrowser™ Data Integration software, facilitating post-acquisition analysis of large volumes of generated data
iCys
iCys® Research Imaging Cytometer
The iCys Research Imaging Cytometer opens new avenues of experimental design for investigations into cellular and tissue-based processes. The ideal platform for generating precision high-content data on a broad range of specimen types, iCys offers flexibility and a powerful
analytical feature set. iCys is the versatile analysis platform that combines the precise quantification of laser-based cytometry, the high content of automated image analysis and the visualization and sample manipulation benefits of inverted microscopy.
Microscope Olympus IX-71 microscope with Brightfield illumination and optional Epi fluorescence illumination kit.
Software iCys Standard Cytometric Analysis Software iBrowser Data Integration Software iNovator Application Development Toolkit
Autofocus
Laser-based autofocus, programmable for any number of standard or custom carrier options and objectives.
Automated Stage
Precise X-Y auto stage with scanning range of0.05 to 20 microns per step.
Magnification
Standard 10X, 20x and 40x objectives Optional 60x
Specimen carriers
Flexible scanning options including: - Glass and plastic microtitre plates from 6 to384 wells - Microscope slides - Chamber slides - Petri Dishes - Custom carriers
Computer
Intel Quad Core Processor, 8GB RAM, Dual Hard drive, 10/100 NIC, DVD-R/W, Windows XP Professional Operating System.
Analytical Performance: Fluorescence precision: within run CVs ~ 2.5%(with Beckman Coulter Flow Check beads) Sensitivity, Resolution: Resolves 8-peak Spherotech "Rainbow" beads Dynamic Range: Three + decades of integrated fluorescence.
Instrument Configurations
#
Detail
Part Number
Description
View
1
A89296
- Four Lasers
Red (633nm Helium-Neon) Yellow (561nm Diode
Pumped Solid State)
Blue (488nm Diode Pumped Solid State)
Violet (405nm Diode)
- Four PMT's and Standard Collection Filters: 440/30, 530/30, 600/50 & 650LP
- Suggested Optional Filters: 510/21 (GFP); 625/30, which can be used in conjunction with the standard 650LP filter when running
the "fruit" fluorescent protein stains (must be purchased
separately)
- Dual Channel Absorption/Scatter Detector
- Variable Resolution Scanning (including high resolution
option)
- IX71 Inverted microscope with Brightfield
- EPI
Fluorescence Light
- Quad Core Computer (w/8GB DDRw), 30" Flat
Panel Wide Screen Monitor
- Robotic Plate Handler Interface,
Positioning Hardware and Serial Interface electronics
- iCys
Standard Cytometric Analysis Software plus iBrowser Data Integration software
2
A89297
Also available in 220V
Instrument Options
#
Detail
Part Number
Description
View
3
A89303
Green Filter Cube (515/30)for use with A89355 and A89356
Remote Workstation Software
#
Detail
Part Number
Description
View
4
A89305
iCys Advanced Remote Workstation Software Module
This option is available only to customers with the iNovator Application Development Tooolkit installed on the iCys Cytometer. It includes:iCys Standard Cytometric Analysis Software; iCys iNovator Application Development Toolkit; iCys iBrowser Data Integration Software
Software Option
#
Detail
Part Number
Description
View
5
A89298
iNovator Application Development Toolkit for iCys's Standard Cytometric Analysis
iNovator Application Development Toolkit is an add-in for iCyte or iCys Cytometric Analysis Software, offering a set of high-leve ltools for developing new application protocols and experimental systems. With ad istinctive user interface and abroad menu of tools for specimen handling and image processing, iNovator opens up additional possibilities in high-content cellular and tissue analysis.
This is one example of the many configurations available for the iCys Research Imaging Cytometer. More configurations, instrument software and hardware options, collection filter cubes, remote workstation options, and accessories are available through your sales representative or on this web site.
iCyte
iCyte® Automated Imaging Cytometer
The iCyte Imaging Cytometer has the flexibility and automation you need to investigate the most complex cellular functions, including precise quantification of DNA and cell cycle information, and localization of multiple cell constituents. iCyte combines digital microscopy, image processing and the population data analysis of analytical cytology to provide the highest information content.
Software iCys Standard Cytometric Analysis Software iBrowser Data Integration Software iNovator Application Development Toolkit
Autofocus
Laser-based autofocus, programmable for any number of standard or custom carrier options and objectives.
Automated Stage
Precise X-Y auto stage with scanning range of0.05 to 20 microns per step.
Magnification
Standard 10X, 20x and 40x objectives Optional 60x
Specimen carriers
Flexible scanning options including: - Glass and plastic microtitre plates from 6 to384 wells - Microscope slides - Chamber slides - Petri Dishes - Custom carriers
Computer
Intel Quad Core Processor, 8GB RAM, Dual Hard drive, 10/100 NIC, DVD-R/W, Windows XP Professional Operating System.
Analytical Performance: Fluorescence precision: within run CVs ~ 2.5%(with Beckman Coulter Flow Check beads) Sensitivity, Resolution: Resolves 8-peak Spherotech "Rainbow"Â beads Dynamic Range: Three + decades of
integrated fluorescence.
Instrument Configurations
#
Detail
Part Number
Description
View
1
A89351
- Four Lasers
Red (633nm Helium-Neon) Blue (488nm Diode Pumped Solid State)
Green (532nm Diode Pumped Solid State)
Violet (405nm Diode)
- Four PMT's and Standard Collection Filters: 440/30, 510/21, 575/50 & 650LP
-
Suggested Optional Filters: 515/30 (which improves Green emission response when not running 532nm laser in same pass (must
be purchased separately)
- Dual Channel Absorption/Scatter Detector
- Variable Resolution Scanning (including high resolution option)
- IX51 Inverted microscope with Brightfield
- Quad Core Computer (w/8GB DDRw), 30" Flat Panel Wide Screen Monitor
- Robotic Plate Handler Interface, Positioning Hardware and Serial Interface electronics
- iCyte Standard Cytometric Analysis Software plus iBrowser Data Integration software
2
A89352
Also available in 220V
Instrument Options
#
Detail
Part Number
Description
View
3
A89303
Green Filter Cube (515/30)for use with A89347, A89348, A89351, A89352
Remote Workstation Software
#
Detail
Part Number
Description
View
4
A89370
iCyte Advanced Remote Workstation Software Module
This option is available only to customers with
the iNovator Application Development Tooolkit installed on the
iCyte Cytometer. It includes:iCyte Standard Cytometric Analysis
Software; iCyte iNovator Application Development Toolkit; iCyte
iBrowser Data Integration Software
Software Option
#
Detail
Part Number
Description
View
5
A89369
iNovator
Application Development Toolkit for iCyte Standard Cytometric
Analysis
iNovator Application Development Tool kit is an add-in for iCyte or iCys Cytometric Analysis Software, offering a set of high-level tools for developing new application protocols and experimental systems. With a distinctive user interface and a broad menu of tools for specimen handling and image processing, iNovator opens up additional possibilities in high-content cellular and tissue analysis.
This is one example of the many configurations
available for the iCyte Automated Imaging Cytometer.
More configurations, instrument software and
hardware options, collection filter cubes, remote
workstation options, and accessories are available
through your sales representative or on this web
site.
iGeneration Software
iNovator Application Development Toolkit
The iNovator Application Development Toolkit adds significantly to the capabilities of both the iCyte and iCys systems. With iNovator, users can:
· Employ advanced image processing tools to the segmentation and data analysis process · Control this process with visually oriented macros · Perform multi-scale scanning and analysis
The iNovator Workspace
iNovator provides a number of image processing tools to aid in
contouring laser scan images. These tools include:
• Image Filters - Image filters can accentuate details in
captured images. The image filters available in iNovator include
High Gauss, High Pass, Low Pass, Vertical Edge and Laplace. These
filters can accentuate certain details in the images ,
including partial deconvolution of the laser beam spread function.
• Morphological Filters - Morphological filters allow images
to be more clearly visualized by turning pixels on and off
according to filtering criteria. Morphological filters include
erosion, dilation, opening, and closing.
• Watershed Segmentation - Watershed segmentation uses
Euclidian distance maps to separate closely spaced or
overlapping events.
Multiple Analysis Paths and Components
Multiple analysis paths may be
defined within a given macro to simultaneously contour
on virtually any number of different event types or
components. The sample iNovator workspace below shows
two paths that use different contouring criteria. The
first path contours on the Green fluorescence channel
and the second path contours on the Orange channel using
a different threshold.
This feature allows two sets of events to be generated during
a single run. In the following graphic, the second
path's events are named "Nuclei" but they can be given
any name and be associated with any structure on which
the analysis path is designed to contour.
Additionally, different event components can be related to one
another by using an inclusion module within the macro.
For example, if an event contour of component type A is
included in the event contour of component type B, then
a relationship can be established between the two.
iNovator Application Development Toolkit
With the iGeneration applications, various types of data files, both numerical and image, can be defined and saved. Each type of data element may have several entities, and iCyte and iCys can obtain a complete set of the data elements for all the samples in an analysis, such as wells in a microtiter plate analysis or core samples in a tissue microarray. iBrowser® Data Integration Software provides a convenient method to access, review, and report on data.
A Run file serves as a "master index" for all data associated with a given run. iBrowser uses the Run file as the key to displaying, analyzing, and reporting on iCyte or iCys Run data.
Displaying Summary Data
The iBrowser software displays summary data for a specific Run, including annotations for that Run, the Run name and ID, and a table of the well features for all wells or scan areas for that Run. Figure 1 shows the portion the Run file for an experiment in the starting window of the iBrowser software. iBrowser displays the experimental annotations, as well as the actual Well Feature data.
iBrowser provides a modified form of the Kolmogorov-Smirnov test, in
which a group of control wells is defined. The test compares each of
the histograms in the data set to the control value and generates a
new D-value histogram.
Data generated on multiple carriers within a single run
(using the iCyte robot plate handler) may be displayed one of two
ways. Data may be displayed on a carrier-by-carrier basis, with the
user selecting which carrierr's data to display for each data
tab. Alternatively, all the wells or scan areas may be displayed
together. This latter option allows comparisons between wells of
different carriers.
Displaying Well Data
Any of the Well Features generated in iCys or iCyte can be graphed as well feature histograms, scattergrams or as 2-parameter histograms (expression maps).
A group of wells or a scan area may be defined so that specified statistics may be applied to data from the entire group. Groups may be substituted for individual wells in the well-data display such that graphical data of
an average of the group members (as opposed to a single well) is displayed.
Reporting iCyte and iCys Findings iBrowser can generate reports from any tab. Additionally, using the Well Report feature, a report can be generated that contains multiple
elements for a single well, such as Well Images, Galleries, and Scattergrams.
Reports can be printed, saved as images, or saved into PDF files.
Presentations
An animated, narrated presentation of the
iGeneration technology. Click on the link
below to download the presentation in the language
of your choice.
For Research Use Only. Not for use in diagnostic procedures
Laser Scanning Cytometry (LSC) uses laser-based opto-electronics and automated analysis capabilities to simultaneously and rapidly measure biochemical constituents and evaluate cell morphologies. LSC technology provides a measure of precision and visualization, combined with flexibility that is available in no other cellular analysis system.
