Beckman Coulter´s complete range of automation and
information systems help you streamline processes for
maximum efficiency. From delivery of more timely,
accurate and reliable patient test results to the
elimination of bottlenecks, our automation and
information system solutions empower you to manage lab
operations more efficiently and cost-effectively. To
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Information request form Fill the short form below to request additional information on Flow Cytometry instrumentation.
A Flow Cytometry team member will contact you within 24 hours.
Submit your information or select another icon to close this form.
For Research Use Only. Not for diagnostic procedures
The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays.
Sensitivity, Resolution and Dynamic Range
Plug and Play Filters
The Gallios Flow Cytometer Features
Available in 3 configurations
6 color 2 Laser (5+1) 8 color 2 Laser (5+3) 10 color 3 Laser (5+3+2)
Innovative laser cartridges
Picomotor driven beam steering optics Laser control to conserve laser life Design for laser flexibility Customer temperature regulation
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Gallios Revealed
Optics in Action
Efficient delivery of light
More power at the flow cell for greater resolution in your applications Efficient and reliable solid state blue, red, and violet lasers Free space delivery of laser light Optimized beam steering optics Increased power at flow cell
Scatter Parameters
Efficient delivery of light 3 unique setting for relative size measurements
Innovative submicron particle resolution setting for forward scatter Independent side scatter alignment
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Inside the Gallios
Electronics Explained
Plug and play filters allow rapid instrument reconfiguration between experiments
Filters are easily accessible and interchangeable with no tools required
Patented electronics design incorporates fast, accurate measurement of signals over a wide range of event rates
Fast pulse recovery Minimize linearity errors Sensitivity of dim particles Minimize dead time Accuracy at high event rates
Instrument Dashboard LEDs clearly indicate instrument status from across the lab
Software features:
Multi-User Administration Drag & Drop replay of data Boolean gating and color eventing hierarchy New log/lin transform for visualization of negative data and compensation optimization Automatic PDF and .xls creation
PROService Compatible
On-line Documentation
To view a document click on one of the icons below.
MoFlo™ XDP Cell Sorter
High speed sorting with proven results
The MoFlo™ XDP The MoFlo™ XDP has set the standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity and with biosafety solutions. This high speed sorter is compatible with array of laser options. High viability, yield, and purity with an analysis rate of 100,000 events per second and sort rates of up to 70,000 events per second are standard. Improved light collection and filter designs optimize the sensitivity. Experience unmatched sorting capabilities while maintaining high performance, biosafety, and modularity.
For Research Use Only. Not for diagnostic procedures
New Features: Electronics are the fastest available IntelliSort Summit Software v5.2 Touch Screen Control Panel
Key Features: High sort yields with preserved cell viability and function Proven sort speeds of up to 70,000 events per second Accurate and reproducible single cell deposition
Additional XDP Benefits: Stable fluidics critical for sorting SmartSampler sample input 4-Way™ sort capability CyClone™ precision unmatched Aerosol Evacuation System Temperature control Sterile tubing kit Expandable laser options
On-line Documentation
To view a document click on one of the
PDF icons below.
Comparison of the Analysis Capabilities of Beckman Coulter MoFlo XDP and Becton Dickinson FACSAria I and II
FC 500 Series
More Flexibility for Your Cytometry Research, the FC 500 Series is a sophisticated product family equipped with the most advanced technologies available.
More Flexibility for Your Cytometry Applications The Cytomics FC 500 Series is a sophisticated product family equipped with the most advanced technologies available. The FC 500 Series delivers flexible testing, powerful software and a user-friendly interface - features that help scientists optimize their overall workflow efficiency while maintaining true cost effectiveness. Plus, each FC 500 Series system is backed by Beckman Coulter's unsurpassed reputation for quality and service.
FC 500 MCL System with CXP Software The FC 500 System provides automated tube based acquisition for cell based assays. Utilizing powerful CXP software, investigators can create custom panel reports and perform multiple tube panel based analysis. The automated QC database allows daily monitoring of instrument QC.
FC 500 MPL System with MXP Software The FC 500 MPL System provides automated plate and tube based acquisition for cell and bead based assays. MXP software allows users to automatically setup assays and rapidly run samples in Microtiter plates for screening and higher throughput analysis.
Product Features
Precise, Flexible Analysis Every Time
Designed for powerful performance and unmatched simplicity
Conducts 5-color analysis from either single or dual laser excitation
Improves efficiency by enabling scientists to analyze more antibodies per tube than ever before on an automated system
Gives investigators the power to expertly manage every aspect of their flow cytometry studies with the revolutionary CXP software
Increased Flexibility with Two Lasers and Multiple Fluorochromes
Equipped with a collinear second laser that maintains a stable alignment with a single beam path and allows greater versatility in antibody and fluorochrome choices
Provides versatility to use either PC5 or APC as the fluorochrome for FL4 in multicolor immunophenotyping applications
Eliminates the need for time delay calculations and the challenges associated with spacially-separated beams
Wavelength independent detectors facilitate application development
Simplified Multicolor Analysis with Advanced Digital Compensation (ADC)
Beckman Coulter's ADC technology provides superior correction of spectral overlap and more accurate results. With ADC technology, biological samples can be used for automated color compensation, without the limitations of latex beads. Only Beckman Coulter flow cytometers offer patented Digital Signal Processing (DSP) for excellent linearity, drift-free amplification and color compensation.
The FC 500 with ADC also enables investigators to:
Perform digital signal processing with 20-bit data in real-time
Obtain fast, intuitive compensation feedback to set or fine-tune compensation manually with the QuickCOMP feature
Increase the visualization of low intensity fluorescence populations
To accommodate numerous configurations, the FC 500's user changeable filters provide fluorochrome selection flexibility. Customized Photomultiplier Tubes (PMTs) detect a broad range of wavelength emissions, which can be used for virtually any dye excited by 488 or 635 nm lasers.
Five-Color Analysis From A Single Laser Has Never Been Easier
Only the FC 500 can be used with a variety of fluorochromes to analyze five colors (FITC, PE, ECD, PC5 or APC and PC7) using either one or two lasers. Investigators could also use Beckman Coulter's IOTest multicolor reagents and drop in their choice of additional conjugated antibodies to make their own combinations.
Performs five-dimensional automated phenotyping analysis with Prism, a unique data reduction display allowing analysis of up to 32 individual phenotypes in a single plot.
Extends multicolor analysis to utilize the full visible emission spectra, which further boosts the level of flexibility that can be achieved
On-line Documentation
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COULTER® PrepPlus™ Series Workstations
Standardize And Improve Productivity From Start To Finish.
Whether your lab needs the automated benefits of the COULTER® PrepPlus Series and COULTER® TQ-Prep -or the cost-effectiveness of the COULTER® Q-Prep workstation- Beckman Coulter has a solution that meets all your sample preparation needs. With automation expertise unmatched in the industry, our complementary systems will help standardize and enhance your entire flow cytometry process.
Product Features
Automate High-Volume Sample Processing, While Enhancing Efficiency and Precision
Connecting a PrepPlus workstation to the TQ-Prep provides your laboratory a single, comprehensive system that automates your entire sample preparation process.
A PrepPlus-controlled by the TQ-Prep through its interactive touch-screen-provides precision pipetting of reagents, patient samples, controls and calibrators into secondary tubes. Plus, lysing is automatically performed on the TQ-Prep, with minimal operator intervention.
The PrepPlus Series Helps Increase Your Lab's Efficiency, Through:
Enhanced precision through automated processing
Increased sample throughput, up to 90 daughter tubes per hour
Flexible software programming of reagents, controls and pipetting parameters
Sample flexibility that provides the ability to run whole blood and body fluids
Improved safety with closed tube sampling, from a wide range of primary vendor specimen collection tubes
Maintained whole blood cell suspensions by means of rotating specimen rack
The PrepPlus 2 Provides Additional Enhancements to Increase Customer Efficiency and Throughput:
Flex-start position for primary sample tube at position 1 or 13 (front or back sample rack)
Onboard carousel sample mix at end of run
Additional open vial position to accommodate multiple size tubes
User service interface touchscreen
Additional Information
To view a document click on one of the icons below.
Automated Cell Viability Analysis Today's research and biologic production demands on cell and tissue culture assumes no guesswork to confirming viable cell populations. Therefore, both cell concentration and percentage of viable cells must be determined accurately and precisely.
The new Vi-CELL Series Cell Viability Analyzers
provide an automatic and cost effective means to perform the
trypan blue dye exclusion method. This data is essential to
the decision making process for basic tissue culture cell
passage and maintaining optimum culture conditions in
bioreactors. Cell and tissue culture applications in life
science research, including recombinant protein and
biopharmaceutical production, require the accuracy,
precision and automation available only in the Vi-CELL cell
viability analyzer.
The Vi-CELL automates the widely
accepted trypan blue cell exclusion method. Historically,
cell viability determinations were performed manually using
a light microscope and hemacytometer. This technique has
major shortcomings due to subjective determination of cell
count, as well as manual, time consuming steps. In
comparison to the manual technique, the Vi-CELL
automatically performs the trypan blue with video imaging of
the flow-through cell. Results are obtained in minutes.
For Research Use Only. Not for diagnostic procedures.
Product Features
Automation of the standard trypan blue assay
% Viability
Total cell concentration
Total viable cell concentration
Mean cell size
Real time cellular images
Bioprocess monitoring
Validated reagents
Convenient reagent pack
On-line Documentation
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BECKMAN-COULTER® Sample Preparation System FP1000 with continuous feeding of samples to the FP1000 Sample Preparation System, any run size proceeds smoothly and rapidly. At the same time, the samples are tracked and verified throughout the process, reducing errors, re-runs and incorrect sample IDs. The user determines which reagents and lytic system to use based on the protocol, and they are loaded into the FP1000. Additionally, the number of mixes can be programmed by the user, facilitating accurate reagent setup results, and delivering unparalleled flexibility.
Volume Range
Pipetting Module (5 μL to 1,000 μL)
Syringe Pump Size
Aspiration/Dispense pump 2mL
Wash/Prime pump 5 mL
IMMUNOPREP™ reagents A, B, C 2 mL
Flow-Count™ pump 2 mL
Specimen Size
5 μL to 1,000 μL (Volumes over 100 μL are delivered in multiple aliquots. Maximum aliquot volume is 100 μL)
Specimen Tube Size
10 to 13 mm tubes
3 mL (10.25 x 64 mm) pediatric tubes
2 mL (13 x 47 or 50 mm) pediatric tubes
Specimen Tube Types
Becton Dickinson Vacutainer* Tubes Worldwide
Becton Dickinson Glass Tubes with Hemogard*
Closure
Sarstedt
Sherwood Medical Tubes (Monoject)
Specimen Cassette Sizes
Two sizes:
13 mm diameter tubes control tubes and pediatric size tubes in adaptors
16 mm diameter tubes
Carousel Tubes (Daughter Tubes)
12 x 75 mm straight polypropylene or polystyrene test tubes
Bar Code Symbologies
(Sample Identification)
Code 128 (USD-6)
Code 39
Interleaved 2 of 5 (also known as 12 of 5, USD-1 and USD-3)
Codabar (also known as USD-4 and NW7)
System Performance Accuracy (by weight)
Specification
Example Study Data
10 μL
9-12 μL
0.011
20 μL
18-22 μL
0.021
50 μL
47-53 μL
0.050
100 μL
95-105 μL
0.098
Precision
Specification (%CV)
Example Study Data(%CV)
10 μL
CV<10%
1.94%
20 μL
CV<10%
2.51%
50 μL
CV<5%
1.73%
100 μL
CV<5%
1.05%
Carryover
Range
Example Study Data
Fluorescence
<1%
0.0%
Primary Blood Sample
<1%
<0.08%
Throughput
>80 daughter tubes per hour Example data: 90 daughter tubes per hour in batch mode with tetraCXP (approx. 3 carousels per hour)
Installation Category - Over voltage Category II Input Voltages - 100/240 Vac Frequency - 48/62 Hz Maximum Power - 150 W
Noise
≤ 65 db
Operating Temperatures
16 to 32° C (60 to 90° F)
Operating Humidity Range
30% to 85%, without condensation
Safety
UL, CSA and CE
Pollution
Degree 2
Altitude
Up to 2,000 m
Workstation Computer
Intel* Pentium* 4 microprocessor
Data Storage
3.5 in, 1.44 MB diskette drive 40 GB hard disk 52x (or higher) CD-ROM drive, MPCII compatible 750 MB ZIP* drive
Interfaces
Windows* 2000 SP4 Bidirectional asynchronous serial interfaces for communication with mainframes and personal computers via Data Innovation's Instrument Manager*
Monitor
SVGA flat-screen 17 inch color monitor
Communications
One parallel port Two USB ports Onboard Ethernet network interface
Input Devices
PS/2 optical IntelliMouse* Standard IBM*-AT compatible 102 keyboard Chumoto hand held bar code reader
On-line Documentation
To view a document click on one of
the icons below.
For Research Use Only. Not for diagnostic procedures
Laser Scanning Cytometry (LSC) uses laser-based opto-electronics and automated analysis capabilities to simultaneously and rapidly measure biochemical constituents and evaluate cell morphologies. LSC technology provides a measure of precision and visualization, combined with flexibility that is available in no other cellular analysis system.
Although LSC technology has a flow cytometry heritage, it is not limited to analyzing cells in fluids. Instead, the technology allows automated analysis of solid-phase samples, including adherent cultured cells, tissue sections, cancer tissue imprints, and cytology smears, preserving the sample along with the exact position of each measured sample. This important feature allows the researcher to automatically return to, visually inspect and interrogate specific cells having defined genetic, biochemical, or morphological properties; or to remeasure specimens after retreating them with reagents or drugs.
Laser Scanning Cytometry allows users to:
· Generate stoichiometric data and analyze heterogeneous cell populations.
· Visualize specimens conventionally with either powerful laser-scanning imaging techniques or with a microscope.
· View any number of individual events in the population data.
· Reanalyze the same cells under varying conditions.
· Measure and localize cellular constituents.
· Detect molecular constituents in the surrounding environment and correlate their presence to cell processes.
· Simultaneously study cultured cells at the individual- and colony-level.
· Perform automated analysis on tissue sections and tissue microarrays.
iGeneration Product line
iCys® Research Imaging Cytometer
The iCys® Research Imaging Cytometer combines the technological advances of the existing LSC and iCyte instruments, providing the ability to visualize the specimen through microscope optics or with a digital camera.
The combination of analytic software advances with microscope features provides the iCys with a unique mode of analysis. Once scanning and analysis are completed, the microscope stage may be automatically moved to the location of any event of interest. The event can then be viewed using the microscope optics while simultaneously viewing the laser scan images. The user may further utilize any number of microscope accessories available, including opto-mechanical devices for micromanipulation and cell capture.
· Simultaneous acquisition of multiple-fluorescence and brightfield laser-scatter images, from as many as 3 lasers and 5 multiplexed light sensors
· Wide-field images, super-wide-field sampling and mosaic images
· Easily viewed specimens with on-the-fly galleries, or relocation to individual events after data acquisition
· The option for either laser scan images or live microscope or camera viewing, or both
· Analysis and visualization of specimens in a variety of formats (chamber slides, microtiter plates, petri dishes, microscope slides) to analyze cell populations, cell colonies, tissues and tissue arrays
· Precise localization of cells and tissue events of interest with the automated motorized stage
· Autofocus for automated analysis
· Easy interface with peripheral microscopy devices like cameras, phase-contrast visualization, micromanipulators, cloning devices, etc
iCyte® Automated Imaging Cytometer
First introduced in 2002, the iCyte® Automated Imaging Cytometer is designed to meet the demand in the biotechnology and pharmaceutical markets for automated high-content cellular analyzers which provide for higher throughput. Among its many advanced features is the ability to precisely measure cellular DNA content - essential in toxicological studies - and simultaneously combine the information gathered from these measurements with other molecular markers and cell morphology. The iCyte´s unique software offers unprecedented flexibility in data analysis.
While the instrument relies on the basic laser scanning technology, its unique features include:
· Morphological and quantitative features for very high content cell-based analysis
· Multiple lasers and photomultipliers for flexible dye combinations
· Compatibility with a variety of carriers, including microtiter plates and microscope slides, for unsurpassed specimen flexibility
· Automated or manual operation modes
· Cellular and tissue specimen analysis
· Open assay development and data reporting platforms to accommodate rapidly changing discovery needs
iCyte enables fully automated, walk-away analysis of cellular specimens. New features include:
· Robotic specimen loader with 45-tray capacity, allowing walk-away experimental analysis
· Optional iNovator™ Application Development Toolkit, enabling fully automated tissue identification and scan region selection. Accurate quantitative measurement of tissue fluorescence is enhanced by novel background fluorescence subtraction techniques
· Flexible iBrowser™ Data Integration software, facilitating post-acquisition analysis of large volumes of generated data
iGeneration Software iNovator Application Development Toolkit
The iNovator Application Development Toolkit adds significantly to the capabilities of both the iCyte and iCys systems. With iNovator, users can:
· Employ advanced image processing tools to the segmentation and data analysis process · Control this process with visually oriented macros · Perform multi-scale scanning and analysis
The iNovator Workspace The iNovator workspace provides multiple modules that control the various functions of data acquisition, image processing, segmentation, and event generation. By inserting these modules into the macro workspace and connecting them together, analysis pathways can be built to control the scanning and data acquisition process.
Image Processing Tools iNovator provides a number of image processing tools to aid in contouring laser scan images. These tools include:
· Image Filters - Image filters can accentuate details in captured images. The image filters available in iNovator include High Gauss, High Pass, Low Pass, Vertical Edge and Laplace. These filters can accentuate certain details in the images , including partial deconvolution of the laser beam spread function.
· Morphological Filters - Morphological filters allow images to be more clearly visualized by turning pixels on and off according to filtering criteria. Morphological filters include erosion, dilation, opening, and closing.
· Watershed Segmentation - Watershed segmentation uses Euclidian distance maps to separate closely spaced or overlapping events.
Multiple Analysis Paths and Components
Multiple analysis paths may be defined within a given macro to simultaneously contour on virtually any number of different event types or components. The sample iNovator workspace below shows two paths that use different contouring criteria. The first path contours on the Green fluorescence channel and the second path contours on the Orange channel using a different threshold.
This feature allows two sets of events to be generated during a single run. In the following graphic, the second path's events are named "Nuclei" but they can be given any name and be associated with any structure on which the analysis path is designed to contour.
Additionally, different event components can be related to one another by using an inclusion module within the macro. For example, if an event contour of component type A is included in the event contour of component type B, then a relationship can be established between the two.
iBrowser® Data Integration Software
With the iGeneration applications, various types of data files, both numerical and image, can be defined and saved. Each type of data element may have several entities, and iCyte and iCys can obtain a complete set of the data elements for all the samples in an analysis, such as wells in a microtiter plate analysis or core samples in a tissue microarray. iBrowser® Data Integration Software provides a convenient method to access, review, and report on data.
A Run file serves as a "master index" for all data associated with a given run. iBrowser uses the Run file as the key to displaying, analyzing, and reporting on iCyte or iCys Run data.
Displaying Summary Data
The iBrowser software displays summary data for a specific Run, including annotations for that Run, the Run name and ID, and a table of the well features for all wells or scan areas for that Run. Figure 1 shows the portion the Run file for an experiment in the starting window of the iBrowser software. iBrowser displays the experimental annotations, as well as the actual Well Feature data.
iBrowser provides a modified form of the Kolmogorov-Smirnov test, in which a group of control wells is defined. The test compares each of the histograms in the data set to the control value and generates a new D-value histogram.
Data generated on multiple carriers within a single run (using the iCyte robot plate handler) may be displayed one of two ways. Data may be displayed on a carrier-by-carrier basis, with the user selecting which carrier's data to display for each data tab. Alternatively, all the wells or scan areas may be displayed together. This latter option allows comparisons between wells of different carriers.
Displaying Well Data
Any of the Well Features generated in iCys or iCyte can be graphed as well feature histograms, scattergrams or as 2-parameter histograms (expression maps).
A group of wells or a scan area may be defined so that specified statistics may be applied to data from the entire group. Groups may be substituted for individual wells in the well-data display such that graphical data of
an average of the group members (as opposed to a single well) is displayed.
Reporting iCyte and iCys Findings
iBrowser can generate reports from any tab. Additionally, using the Well Report feature, a report can be generated that contains multiple
elements for a single well, such as Well Images, Galleries, and Scattergrams.
Reports can be printed, saved as images, or saved into PDF files.
Quantitative Imaging Cytometry
An animated, narrated presentation of the iGeneration technology. Click on the link below to download the presentation in the language of your choice.
Available in these languages:
Reports can be printed, saved as images, or saved into PDF files.
Quantitative Imaging Cytometry
An animated, narrated presentation of the iGeneration technology. Click on the link below to download the presentation in the language of your choice.
Available in these languages:
To
download a presentation click on one of the icons below.
TQ-Prep™ Workstation An efficient sample preparation system for flow cytometry
The TQ-Prep™ Workstation enables today's clinical laboratories to meet heavier immunophenotyping and CD4 measurement demands. The TQ-Prep Workstation and Beckman Coulter's ImmunoPrep Reagent Systems are perfect partners, providing extremely reliable operation with precise, accurate cell lysing and fixation.
Product Features
You have flexibility
Choose between the standard ImmunoPrep Reagent System configuration or the optional low angle light scatter reagent configuration according to your flow cytometer needs
Choose between on or off-line incubation step to maximize walk-away capability
Choose between set-up on a bench top or inside a safety cabinet to suit your safety considerations
You have high reliability
Each component has been tested in excess of 100,000 cycles
A new syringe pump system dispenses reagents in precise and accurate volumes
Vortex system mixing ensures reproducible lysis of homogenous cells
You have convenience
Operation is simple with universal touch screen icons
You receive constant feedback as to where you are in the incubation and lysing process
You have virtually maintenance-free operation
Your reagent use is monitored by robust conductive reagent sensing and cycle counters
Maximizes the resolution of cell populations to allow easy identification either
by forward scatter or low angle light scatter
Excellent versatility
The TQ-Prep Workstation and ImmunoPrep Reagent System
are directly compatible with the Coulter EPICS XL-MCL flow cytometer and
can also operate with any flow cytometer. The system provides up to 24 hour post preparation stability.
High Speed with Accuracy and Reproducibility
Provides the fastest no-wash flow cytometry-ready preparation available today
Takes a sample from whole blood to lysis in seconds
Maintains cellular integrity without distortion
Minimizes debris or platelet adhesion to leukocytes through clean sample preparations
For Multi-Q-Prep or TQPrep Workstation, part no.
7546999
Products are available "For Research Use Only. Not
For Use In Diagnostic procedures" (RUO) unless indicated "For In Vitro
Diagnostic Use" (IVD) or "Analyte Specific Reagent -- Analytical and performance
characteristics are not established" (ASR).
The CyAn ADP Analyzer is the next step in Advanced Digital Processing
Enhanced detection for flow cytometry
State-of-the-art optics and electronics
Automated compensation
Easy to walk-up operation
We offer two models to accomodate a diverse range of applications, with up to 11 standard parameters and 9 colors. CyAn ADP 7 Color has 9 parameters (FSC, SCC,
FL1-FL7) and is equipped with 488 nm and
642 nm solid-state lasers. Some key research
applications include CD34 enumeration, signal
transduction, antigen-specific T-cell detection and
multicolor immunophenotyping. CyAn ADP 9 Color adds the 405nm solid state
laser and two fluorescent channels. Additional
research applications include stem cell/SP
analysis, DAPI Cell Cycle analysis and CFP-YFP
FRET analysis.
For Research Use Only. Not for use in diagnostic procedures
On-line Documentation
To view a document click on one of the PDF icons below.
Cell Lab Quanta™ SC MPL The innovative Multi-Platform Loader option for the advanced Quanta SC flow cytometer
It's never been simpler to meet your cell analysis needs. The accurate and efficient Cell Lab Quanta SC MPL flow cytometer provides microtitre plate and micro tube based analysis, with 3-color, Coulter Volume and side scatter analysis for unsurpassed precision and time savings. With its innovative Multi-Platform Loader and AutoSetup capability, you can now use sample cups, micro centrifuge tubes and 24-, 96- or 384-well plates on the same system. Side cup holder contains sample or reagent. Probe wash is automatic and the automatic sample preparation, including incubation, saves time and eliminates human errors. Choose from intuitive, flexible software that offers incremental parameter controls for precise data collection and richer analysis. Optional 21 CFR Part 11 compliance software is also available.
Product Features
Included within the MPL option are two new software packages: Cell Lab Quanta Collection Software for Instrument control, Data Acquisition and Real Time Analysis Cell Lab Quanta Analysis software for Data retrieval and Analysis
Database for data and instrument setting storage
Sample preparation: automatic addition of reagents and mixing prior to acquisition
Real-time data analysis and gating
Real-time and offline listmode compensation
Precise absolute counts
Auto rinse between samples
Auto Start-up and Shut Down Wizards
Plate monitoring function for flagging samples that meet defined criteria
Reactive flagging for well re-run after rinse, flush or clean cycles
Multi-file analysis, including listmode compensation, region settings, overlay analysis plot
User definable statistics for display and exporting as an Excel file
Customizable reports with automated PDF or printer output
COULTER® EPICS® XL™ and XL-MCL™ Flow Cytometer This unique system redefines the standards of flow-cytometric analysis.
The flow cytometer combines the analytical power of a research cytometer into a compact, truly walk away, clinical analyzer ergonomically designed to expedite sample throughput.
EPICS Options & Accessories
The XL system features the capability to analyze up to 4 colors of immunofluorescence from a single air cooled laser. Other multi-color applications include: multiparametric DNA analysis, platelet studies, reticulocyte enumeration, cell biology/functional studies and as well as a broad range of research applications. The instrument is self contained and biohazard safe. The user retains the flexibility to change filter elements for versatility in research settings.
For high volume users, the XL-MCL system offers walk away sample handling for superior efficiency with the Multi Carousel Loader (MCL). Capable of throughputs of up to 100 samples per hour, the fully featured XL-MCL incorporates positive bar-code identification and true vortex mixing prior to aspiration.
The XL remains the only flow cytometer to offer state-of-the-art Digital Signal Processing (DSP) for reliable linearity and drift-free amplification and compensation. The single laser design eliminates concerns regarding multi-beam stability, signal delay and alignment. Compensation is achieved by a unique digital compensation matrix.
The XL SYSTEM II™ software fully automates instrument set-up and compensation for 2, 3 and even 4-color applications. Optional features in the software include tetraONE, the 4-color, one tube, automatic analysis algorithm and reticONE, the automatic analysis algorithm which revolutionizes reticulocyte analysis. Other SYSTEM II features include complete network connectivity with LANtastic Software allowing patient work list and report generation from remote sites. The industry standard SQL/ODBC database allows connectivity to 3rd party, Windows-based, software programs. Plus, true walk away automation is achieved with the AutoPrime feature.
The XL is available in 3 or 4-color configurations, with or without the MCL. Ask your Beckman Coulter representative about the configuration that best meets your laboratory's needs.
Z™ Series COULTER COUNTER® Cell and Particle Counter
Z2™ COULTER COUNTER®
Cell and Particle Counter
uses the Coulter Principle
(Electrical Sensing Zone Method)
for sizing and counting particles
Size Distribution Capability
The Z2 COULTER COUNTER is the latest addition to the Z™ Series COULTER COUNTER. In addition to reporting both count and concentration results, the Z2 adds the ability to provide size distribution of the cell population. The Z2, like the Z1™, uses the
Coulter
Principle (Electrical Sensing Zone Method) of counting and sizing of cells. This technique provides accuracy, speed, versatility and reproducibility. The Z2 displays the entire size distribution graph, or the size statistics and counts between user selectable areas of the graph. In addition, this instrument displays the cumulative count and the cumulative number % above and below a size determined by the cursor positioned on the graph.
Additional Benefit, Averaging Data
The Z2 has the ability to average counts and channelyzed data from a series of up to 10 consecutive analyses. The more cells counted and sized the greater the statistical confidence in the result and the smoother the shape of the averaged size distribution.
PC Data Acquisition
The Beckman Coulter Windows based AccuComp® software package is available for quick and accurate calculation and presentation of sample data statistics from the Z2. Features of the AccuComp software include: size distribution statistics, data archiving, size trend analysis, result overlays, averages, graphs and tables, customizable reports, data import/export capability.
Cell Counting
Both Z1 Single and Dual Threshold Models use the
Coulter
Principle (Electrical Sensing Zone Method) for counting
cells or particles in the 1-120 micron size range. This
technique provides accuracy, speed, versatility, and excellent
precision; it is the accepted reference method for cell
counting. Standard apertures are available in five sizes. In
addition, for vey small sample volumes, ampoule insertable
apertures may be used.
The Z1 Single Threshold Model
The Z1 Single Threshold instrument allows the operator to set one size threshold. Cells equal to or larger than this setting will be counted. Ideal as a fully automated instrument for the rapid enumeration of both tissue culture and blood cells from most any species. Excellent for rapid total cell count on cell lines containing cells of relatively uniform size.
The Z1 Dual Threshold Model
The Z1 Dual Threshold Model counts cells or particles in three regions, equal to or above the operator selected lower size, above the upper size setting and between the two sizes. Excellent when cell numbers between a selected size range are required. Instrument of choice when working with numerous cell types, containing variable cell sizes, or platelets.
Product Features
Mercury Free Absolute cell counts or concentration One-Button Calibration Small Footprint Certified to ISO 9001 by NSAI Quality Assurance Technology defined by the International Standard (ISO 13319:2000:
Determination of particle size distributions -- Electrical sensing zone
method) Listed by ETL Testing Laboratory Store up to 5 analyses settings (Profiles) Operator selectable size settings
Specifications
Z2 COULTER COUNTER
Z2 Measurement Range:
1-120 micron overall range
1-60 microns using ampoule insertable aperture tubes
NOTE: Z Series
Starter Kit ( USA- P/N
8320310, Outside USA - P/N
8320311), Z-Series Tri-Pak P/N:8320312,
L10 Standard P/N:6602796,
COULTER CLENZ P/N:8546930
and Aperture Concentration Control P/N:177495
necessary for installation and operation. On the Z Series COUNTER
COUNTER Consumables page, see Reagents & Controls
in the table below for additional information.
NOTE: Z Series
Starter Kit (USA- P/N
8320310, Outside USA - P/N
8320311), Z-Series Tri-Pak P/N:8320312,
L10 Standard P/N:6602796,
COULTER CLENZ P/N:8546930
and Aperture Concentration Control P/N:177495
necessary for installation and operation. On the Z Series COULTER
COUNTER Consumables page, see Reagents & Controls
in the table below for additional information.
Weights and Measures
Z2 COULTER COUNTER
Weight:
13.5 kg (30 lbs)
Height:
44.5 cm (17.5 in.)
Width:
26.5 cm (10.4 in.)
Depth:
35.5 cm (14.0 in.)
Keypad/Display:
6.4 cm (2.5 in.) x 15.5 cm (6.1 in.)
x 15.1 cm (5.9 in.)
Keypad/Display Weight:
0.7 kg (1.5 lbs)
Fill/Waste Station:
19 cm (7.5 in.) x 28.5 cm (11.2 in.)
x 16.5 cm (6.5 in.)
Fill/Waste Station Weight:
1.3 Kg (2.9 lbs)
Z1 Series COULTER COUNTER
Weight:
13.5 kg (30 lbs)
Height:
44.5 cm (17.5 in.)
Width:
26.5 cm (10.4 in.)
Depth:
35.5 cm (14.0 in.)
Keypad/Display:
6.4 cm (2.5 in.) x 15.5 cm(6.1 in.)
x 15.1 cm (5.9 in.)
Keypad/Display Weight:
0.7 kg (1.5 lbs)
Fill/Waste Station:
19 cm (7.5 in.) x 28.5 cm (11.2 in.)
x 16.5 cm (6.5 in.)
Fill/Waste Station Weight:
1.3 Kg (2.9 lbs)
Parts and Accessories
These items were specifically designed for use with a Z Series COULTER COUNTER instrument. Although intended for use with the Z Series COULTER COUNTER, some items can be used with other analytical instruments.
These items were specifically designed for use with a Z Series COULTER COUNTER instrument. Although intended for use with the Z Series COULTER COUNTER, some items can be used with other analytical instruments.
COULTER® PrepPlus™ Series Workstations Standardize And Improve Productivity From Start To Finish.
COULTER Sample Preparation Systems
Whether your lab needs the automated benefits of the COULTER® PrepPlus Series and COULTER® TQ-Prep ¾or the cost-effectiveness of the
COULTER® Q-Prep workstation¾Beckman Coulter has a solution that meets all your sample preparation needs. With automation expertise unmatched in the industry, our complementary systems will help standardize and enhance your entire flow cytometry process.
Connecting a PrepPlus workstation to the TQ-Prep provides your laboratory a single, comprehensive system that automates your entire sample preparation process.
A PrepPlus-controlled by the TQ-Prep through its interactive touch-screen-provides precision pipetting of reagents, patient samples, controls and calibrators into secondary tubes. Plus, lysing is automatically performed on the TQ-Prep, with minimal operator intervention.
The PrepPlus Series Helps Increase Your Lab's Efficiency, Through:
Enhanced precision through automated processing
Increased sample throughput, up to 90 daughter tubes per hour
Flexible software programming of reagents, controls and pipetting parameters
Sample flexibility that provides the ability to run whole blood and body fluids
Improved safety with closed tube sampling, from a wide range of primary vendor specimen collection tubes
Maintained whole blood cell suspensions by means of rotating specimen rack
On-line Documentation
To view a document click on one of the PDF icons below.
Cell Lab Quanta™ SC Advanced Flow Cytometry with 3-Color, Coulter Volume and Side Scatter Analysis
Developed from a unique combination of ingenuity and high-level technical resources, the Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors to provide unsurpassed population resolution and accurate cell counting. This advanced flow cytometer has multiple excitation wavelengths, including 488 nm laser and a UV light source optimized for excitation at 366, 405, 435 nm. Together with interchangeable filters, the Quanta SC allows flexible fluorochrome selection for a variety of multicolor applications typically only achievable with expensive and complicated systems. Side scatter and Coulter volume make size analysis and fluorescent measurements more precise. Unique user features expand ease-of-use and flexibility. When all the details are added together, the sum is performance.
Now Available - Increase Throughput with the
Quanta SC MPL (Multi-Platform Loader) Option
Cell Lab Quanta SC
Product Features
Database for data and instrument setting storage
Sample preparation: automatic addition of reagents and mixing prior to acquisition
Syringe mechanism for precision enumeration
Electronic volume for accurate sizing
Three-color fluorescence detection
Side scatter for granularity measurements
Multicolor excitation for flexible fluorochrome selection
Interchangeable filter block for optimum performance
Cell Lab Quanta SC Software package
Cell Lab Quanta SC Specifications
Cell Lab Quanta SC Flow Cytometer
Absolute Count Performance*
Accuracy: ±5%
Reproducibility (CV): < 5%
Concentration: 3 x 104 to 2 x 106
Fluorescence Performance Specifications
Data acquired using Digital Signal processing and displayed on user selectable
linear or 4 decade logarithmic scales. Resolution: < 3.0 %
HPCV (using TRBC Calibration Standard stained with DAPI and utilizing the mercury arc lamp)
< 3.0 % HPCV (using Flow-Check™ Fluorospheres utilizing the 488 nm diode laser)
Sizing Performance
Electronic impedance with a measurement
range of 3.0 to 40 microns diameter with the standard 125 µm
flow cell.
Optics Performance
Excitation:
Mercury arc excitation optimized at 366, 405, and 435
488 nm laser diode excitation, 2-22 mW user adjustable
Fluorescence Detectors:
3 broad range ultra sensitive photomultiplier tubes
Optical Alignment:
Computer assisted and controlled
Optical Coupling:
1.25NA oil immersion objective
Emission Filters:
Standard Set 460 BP, 525BP, 575BP and 670 LP
Parameters
Electronic Volume, side scatter, time, and 3 color
detection with log/linear and FC/FSD options
Software
Cell Lab Quanta SC Software for Instrument Control,
Data Acquisition and Data Analysis
Fluidics Specifications
Flow Cell Patented 125 µm triangular flow cell (standard)
Sample rate: 4.17µL to 100 µL per minute for 125 µm flow cell
Delivery system: Vacuum pump/motorized syringe pump
Minimum sample size: 150 µL
Maximum sample size: 2 mL
Installation Requirements
Power: 100 V, 120 V, or 220 V, 50/60 Hz
Wattage consumed: 500 watts (total system)
Operating temperature: 60°F to 85°F (16°C to 29°C)
Physical dimensions:
22 W x 26 L x 18 H (in.)
55.9 W x 67.3 L x 45.7 H (cm.)
Instrument weight: 77 lbs (34.9 kg)
*These specifications were validated with an internal 10 µm bead that had an assigned count value. Your results may vary depending on your application and/or sample type.
On-line Documentation
To view a document click on one of the icons below.
For the first time, the Integrated Cytometry Solution offers a seamless pre-analytical, analytical, and post-analytical solution coupled with complete LIS connectivity. By standardizing the five major steps a sample undergoes - and managing the electronic communications between each one - it delivers a progressive automated system that simplifies and streamlines workflow processes, increases efficiency and accuracy, accelerates throughput, and minimizes operator involvement.
Product Features:
For the first time, the Integrated Cytometry Solution offers a seamless pre-analytical, analytical, and post-analytical solution coupled with complete LIS connectivity. By standardizing the five major steps a sample undergoes - and managing the electronic communications between each one - it delivers a progressive automated system that simplifies and streamlines workflow processes, increases efficiency and accuracy, accelerates throughput, and minimizes operator involvement.
Receive
THE SAMPLE ID, test request and demographic information are sent from the LIS and captured in the Information Manager. At the same time, the run information is sent to the FP 1000 database. Complex panels, protocols, reagents and procedures are automatically set up, and a worklist is generated. To ensure accurate handling, data is transferred electronically from LIS to final results. This virtually eliminates transcription errors and costly sample re-runs, while freeing valuable techs for more important tasks.
Prepare
BARCODE tracking on the daughter tubes provides paperless positive ID throughout the process, while three carousels deliver high throughput for increased lab efficiency (up to 96 daughter tubes per hour in batch mode for tetraCXP). For reduced operator involvement and increased safety, the FP 1000´s unique gripping arm transports sample carousels from storage to processing. By accessing sample tubes through cap piercing - as well as automatically dispensing antibodies into the daughter tubes - technologists are protected from potential sample exposure and reproducibility is increased.
Acquire
WITH THE SAMPLES PROPERLY PREPARED, the carousels are moved to the FC 500. The user locates the external worklist file on the FP 1000, and opens it, populating the CXP Acquisition Manager for processing and barcode-based sample tracking. For enhanced flexibility in labs with multiple instruments, the worklist can be sent via ethernet. It can also use native FC 500 or Excel formats, or a combination. To permit the operator to easily work with listmode data during analysis, it´s stored in a user-created directory. With auto-setup capability, multi-color analysis using either single or dual laser excitation, and the ability to use a variety of dyes, the award-winning FC 500 delivers unmatched versatility.
Analyze
REALTIME OR LISTMODE DATA HANDLING, point-click gating, and 20-bit data acquisition are just a few of the ways the innovative FC 500 and its revolutionary CXP software deliver accurate and easy-to-interpret results - all of which can be printed at the FC 500 or uploaded to the LIS.
Report
FOR THE UTMOST FLEXIBILITY AND SAFETY in how information is transmitted, password-protected results can be reviewed and verified at the Information Manager workstation before uploading, or they can be passed on directly to the LIS.
For Research Use Only. Not for diagnostic procedures
Modern flow cytometers are capable of analyzing cells at rates of tens of thousands of cells per second and make many sensitive measurements per cell. There is an abundance of information that is acquired very rapidly on a single cell basis.
To achieve high throughput on a multiple sample or microplate level, samples must be introduced to the flow cytometer rapidly and the resulting data managed efficiently. HyperCyt is the first system that addresses both the front end sample handling and the back end data
analysis for flow cytometers. CyAn ADP, Beckman Coulter's high-performance flow cytometer uses multiple laser excitation sources to analyze biological cells, beads or other microscopic particles. It's state-of-the-art optics utilize high performance
digitalization with pulse processing speeds up to 70,000 events per second complementing a high acquisition and analysis capability of up to 100,000 events. Integrating HyperCyt with the CyAn ADP or your flow cytometer will give you the speed you need to accelerate your research.
HyperCyt primes the sample line going into the flow cytometer with buffer, then samples each well in the microplate. Between each well, it introduces a programmed air gap that separates each specimen.
This series of samples and air gaps are introduced into the flow cytometer in a highly controlled fashion. The data from the entire plate are collected and stored in a single FCS data file.
The HyperCyt System hardware and software to put your flow cytometer into hyperdrive.
Automation friendly
HyperCyt can be integrated with robotic plate loaders
through optional automation interface software.
Rapid sample introduction
HyperCyt can sample up to 40 wells per minute. That means that a 96-well plate can be analyzed in as little as 2½ minutes, a 384-well plate in 10 minutes.
Very small to very large sample volumes
There is no dead volume in the HyperCyt process. This allows you to utilize scarce specimens and get more information than ever before possible. The actual sample uptake can be as little as 1µ, so that kinetic studies on individual wells are possible.
If larger samples are required, HyperCyt can sample for as long as you need.
Utilize standard microplates
You can use standard 96- or 384-well microplates. Even 96-well formatted bullet tubes can be utilized for large sample requirements.
Sampling
HyperCyt control software allows you to select the order in which to sample the plate. Alternatively, you can also select individual wells to sample from the plate.
Mixing
HyperCyt incorporates an optional on-board orbital plate shaker that you can program for your application. You can control the speed, time and frequency of mixing throughout the sampling process.
Rinsing
HyperCyt gives you the option to program rinse steps in order to eliminate carryover whenever that is an issue. Four rinse stations can be used for rinsing or cleaning.
Published results have indicated that carryover is generally 1-2% with no interwell washes.*
*High Throughput Flow Cytometry: Validation in Microvolume Bioassays, Ramirez, Aiken, Andzejewski, Sklar and Edwards, Cytometry Part A S31:55-65 (2003)
Click
on the icon once again to collapse the page. To
purchase parts, accessories or consumables listed for each instrument
click on the part number link or the shopping cart icon
for that respective part. Click
