On-line store:

0 items on your shopping list







Advancing the Science of Cytometry
CYTO 2010  Please Join us for a Beckman Coulter Cellular Analysis Symposia
This event is hosted by the MD Anderson Flow Cytometry and Cellular Imaging Core Facility, an NCI Cancer Center Support Grant funded.
April 22, 2010
MD Anderson Cancer Center
Onstead Auditorium S3.8012 BSRB Building
1515 Holcombe Blvd.
Houston, TX
For additional information please contact John Norman at 281-389-3594 or john.norman@beckman.com.  Click on the icon to open or download the agenda for the Beckman Coulter Cellular Analysis Symposia  .  Click here or on the icon to create an email reminder of this event.Create an email reminder
9:00 - 9:45am The Perfect 10: A technology overview of Beckman Coulters new multicolor solution for flow cytometry red arrow
John Norman, Flow Cytometry, Beckman Coulter, Inc
Abstract:
The development of commercial flow cytometers capable of simultaneously detecting 10 fluorescent signals presents manufacturers with significant challenges. A combination... more

Full Description:
The development of commercial flow cytometers capable of simultaneously detecting 10 fluorescent signals presents manufacturers with significant challenges. A combination of factors receives consideration during the early stages of development to ensure that performance benchmarks are ultimately achieved. This discussion will provide a detailed description of the critical flow cytometric design elements and their collective rolls in optimizing system performance to effectively monitor the dim, high-content, low-frequency rare event targets of the future.


    Collapse window 
Will you be attending this meeting? Yes No

9:45 - 10:30am Signal transduction analysis by flow cytometry in early phase clinical trials of targeted agents treating AML patients red arrow
David Hedley, M.D., Ontario Cancer Institute/Princess Margaret Hospital Dept Medical Oncology and Hematology
Abstract:
Although the analysis of signaling pathways by flow cytometry using phosphospecific antibodies was first developed 10 years ago, this is still considered to be a...more

Full Description:
Although the analysis of signaling pathways by flow cytometry using phosphospecific antibodies was first developed 10 years ago, this is still considered to be a technically demanding application and there are relatively few reports where this has been successfully applied to AML patient samples. An additional challenge using these techniques for pharmacological monitoring is the need for whole blood processing in order to maintain drug/target equilibrium during sample preparation. Our group has made an extensive study of the technique, and has optimized protocols based on combined staining for four activation sites relevant to AML (ERK, Akt, STAT5, and S6), combined with surface markers and light scatter. This protocol gives excellent signal-to-noise for all 4 phosphospecific antibodies, and is able to detect blasts cells down to 0.1% of the total. It is sensitive to the detection of pharmacodynamic effects of targeted agents during clinical trials treating AML patients. Interestingly, it can also detect constitutive signalling involving various pathways in peripheral blood samples from the majority of AML patients, indicating that aberrant pathway activation can persist as these cells leave the bone marrow environment and enter the circulation. The clinical significance of these findings is currently under investigation.


    Collapse window 
Will you be attending this meeting? Yes No

10:45 - 11:30am Laser Scanning Cytometry: An Expanding Role in Biomedical Analysis red arrow
William G. Telford Ph.D. Associate Scientist, Experimental Transplantation and Immunology Branch, National Cancer Institute - NIH
Abstract:
Image cytometry (also termed laser scanning cytometry) represents a unique hybrid technology between flow cytometry and traditional fluorescent microscopy. Imaging cytometers... more.

Full Description:
Image cytometry (also termed laser scanning cytometry) represents a unique hybrid technology between flow cytometry and traditional fluorescent microscopy. Imaging cytometers analyze the light scattering and fluorescent properties of biological specimens like a flow cytometer, but also collect correlated quantitative images of the cells simultaneously. This powerful technique is a dramatic expansion of traditional cytometric analysis, allowing the cell images and their morphological and structural information to become a cytometric parameter, like forward scatter or fluorescence. In this introductory talk, we will discuss the theory and practice of image cytometry and provide a general overview of the technology available to biomedical scientists. We will also cover a wide variety of applications for this technology, from individual cells to intact tissues and whole organisms.


    Collapse window 
Will you be attending this meeting? Yes No

Workshop Description:
Image cytometry (also termed laser scanning cytometry) represents a unique hybrid technology between flow cytometry and traditional fluorescent microscopy. Imaging cytometers analyze the light scattering and fluorescent properties of biological specimens like a flow cytometer, but also collect correlated quantitative images of the cells simultaneously. This powerful technique is a dramatic expansion of traditional cytometric analysis, allowing the cell images and their morphological and structural information to become a cytometric parameter, like forward scatter or fluorescence. In this introductory talk, we will discuss the theory and practice of image cytometry and provide a general overview of the technology available to biomedical scientists. We will also cover a wide variety of applications for this technology, from individual cells to intact tissues and whole organisms.

    Collapse window 

11:30 - 12:15pm Panel Design: Getting More "Multi" into your Multi-Color red arrow
William Godfrey, Ph.D., Manager, Reagent & Application Development, Beckman Coulter, Inc.
Abstract:
In recent years flow cytometers have become more powerful with the addition of large laser palates and multiple flourochrome options. This talk will look at... more.

Full Description:
In recent years flow cytometers have become more powerful with the addition of large laser palates and multiple flourochrome options. This talk will look at the considerations a researcher needs to make while designing high color applications. We will look at the importance of titration, spectral overlap, dye selection, tandem dyes, background control and sample prep when constructing a complex cocktail or panel. Specific panel and cocktail examples to be discussed include L/L, Cell Signaling and NK. We will also examine how any new dyes will effect panel development.


    Collapse window 
Will you be attending this meeting? Yes No

Workshop Description:
Image cytometry (also termed laser scanning cytometry) represents a unique hybrid technology between flow cytometry and traditional fluorescent microscopy. Imaging cytometers analyze the light scattering and fluorescent properties of biological specimens like a flow cytometer, but also collect correlated quantitative images of the cells simultaneously. This powerful technique is a dramatic expansion of traditional cytometric analysis, allowing the cell images and their morphological and structural information to become a cytometric parameter, like forward scatter or fluorescence. In this introductory talk, we will discuss the theory and practice of image cytometry and provide a general overview of the technology available to biomedical scientists. We will also cover a wide variety of applications for this technology, from individual cells to intact tissues and whole organisms.

    Collapse window 

Cellular Analysis Symposia registration Create an email reminder Click here to create an email reminder of this event.
Please fill out the following information:

Name

Mailing Address

City

State/Prov

Zip/Postal Code (required)

Country

Email Address (required)

Lab or Institution

Phone

Add me to your mailing list

 Yes No
Additional comments:
*How did you hear about this event?
Email Colleague Other

Quick Search
Shopping Tools



Reagents


Research Tools


Information Tools


Learning Tools


Visit the Beckman Coulter corporate site

Home | Terms of Use | Contact Us | On-line Store | Admin | Feedback



Powered by CoulterFlow eCommerce 2012 ©